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yeast chromosome pfg markers  (Bio-Rad)


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    Bio-Rad yeast chromosome pfg markers
    Large segments of the mouse X chromosome are specifically targeted with in vitro CRISPR. ( A ) The specific products of the in vitro Otc CRISPR digestion of both YAC ADK.A6 DNA and mouse genomic DNA are resolved by PFGE and detected with Diamond stain (Promega) . Whereas the expected YAC digestion products are clearly detectable, digestion of the more complex mouse genomic DNA does not produce detectable products. Untreated and Cas9-only digestions of YAC and mouse genomic DNA serve as negative controls. ( B ) A Southern blot of the <t>PFG</t> shown in (A) is hybridized with a DIG-labeled Otc probe. The intact YAC chromosome (∼520 kb) and the expected 263 kb in vitro Otc CRISPR digestion product from both YAC and mouse genomic DNA are visible upon chemiluminescent detection of the probe and exposure of X-ray film. ( C ) A faint but, detectable ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is resolved by PFGE along with the multi-megabase H. wingei <t>(Hw)</t> <t>chromosomes</t> ladder (Bio-Rad) labeled on the left. ( D ) The Southern blot of the PFG shown in ( C ) is hybridized with a DIG-labeled Srsx probe. The estimated ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is visible upon chemiluminescent detection of the probe and exposure of X-ray film.
    Yeast Chromosome Pfg Markers, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yeast+chromosome+pfg+markers/pmc05737698-66-7-11?v=Bio-Rad
    Average 90 stars, based on 1 article reviews
    yeast chromosome pfg markers - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "CRISPR-mediated isolation of specific megabase segments of genomic DNA"

    Article Title: CRISPR-mediated isolation of specific megabase segments of genomic DNA

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkx749

    Large segments of the mouse X chromosome are specifically targeted with in vitro CRISPR. ( A ) The specific products of the in vitro Otc CRISPR digestion of both YAC ADK.A6 DNA and mouse genomic DNA are resolved by PFGE and detected with Diamond stain (Promega) . Whereas the expected YAC digestion products are clearly detectable, digestion of the more complex mouse genomic DNA does not produce detectable products. Untreated and Cas9-only digestions of YAC and mouse genomic DNA serve as negative controls. ( B ) A Southern blot of the PFG shown in (A) is hybridized with a DIG-labeled Otc probe. The intact YAC chromosome (∼520 kb) and the expected 263 kb in vitro Otc CRISPR digestion product from both YAC and mouse genomic DNA are visible upon chemiluminescent detection of the probe and exposure of X-ray film. ( C ) A faint but, detectable ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is resolved by PFGE along with the multi-megabase H. wingei (Hw) chromosomes ladder (Bio-Rad) labeled on the left. ( D ) The Southern blot of the PFG shown in ( C ) is hybridized with a DIG-labeled Srsx probe. The estimated ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is visible upon chemiluminescent detection of the probe and exposure of X-ray film.
    Figure Legend Snippet: Large segments of the mouse X chromosome are specifically targeted with in vitro CRISPR. ( A ) The specific products of the in vitro Otc CRISPR digestion of both YAC ADK.A6 DNA and mouse genomic DNA are resolved by PFGE and detected with Diamond stain (Promega) . Whereas the expected YAC digestion products are clearly detectable, digestion of the more complex mouse genomic DNA does not produce detectable products. Untreated and Cas9-only digestions of YAC and mouse genomic DNA serve as negative controls. ( B ) A Southern blot of the PFG shown in (A) is hybridized with a DIG-labeled Otc probe. The intact YAC chromosome (∼520 kb) and the expected 263 kb in vitro Otc CRISPR digestion product from both YAC and mouse genomic DNA are visible upon chemiluminescent detection of the probe and exposure of X-ray film. ( C ) A faint but, detectable ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is resolved by PFGE along with the multi-megabase H. wingei (Hw) chromosomes ladder (Bio-Rad) labeled on the left. ( D ) The Southern blot of the PFG shown in ( C ) is hybridized with a DIG-labeled Srsx probe. The estimated ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is visible upon chemiluminescent detection of the probe and exposure of X-ray film.

    Techniques Used: In Vitro, CRISPR, Staining, Southern Blot, Labeling



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    Large segments of the mouse X chromosome are specifically targeted with in vitro CRISPR. ( A ) The specific products of the in vitro Otc CRISPR digestion of both YAC ADK.A6 DNA and mouse genomic DNA are resolved by PFGE and detected with Diamond stain (Promega) . Whereas the expected YAC digestion products are clearly detectable, digestion of the more complex mouse genomic DNA does not produce detectable products. Untreated and Cas9-only digestions of YAC and mouse genomic DNA serve as negative controls. ( B ) A Southern blot of the <t>PFG</t> shown in (A) is hybridized with a DIG-labeled Otc probe. The intact YAC chromosome (∼520 kb) and the expected 263 kb in vitro Otc CRISPR digestion product from both YAC and mouse genomic DNA are visible upon chemiluminescent detection of the probe and exposure of X-ray film. ( C ) A faint but, detectable ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is resolved by PFGE along with the multi-megabase H. wingei <t>(Hw)</t> <t>chromosomes</t> ladder (Bio-Rad) labeled on the left. ( D ) The Southern blot of the PFG shown in ( C ) is hybridized with a DIG-labeled Srsx probe. The estimated ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is visible upon chemiluminescent detection of the probe and exposure of X-ray film.
    Yeast Chromosome Pfg Markers, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yeast+chromosome+pfg+markers/pmc05737698-66-7-11?v=Bio-Rad
    Average 90 stars, based on 1 article reviews
    yeast chromosome pfg markers - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Large segments of the mouse X chromosome are specifically targeted with in vitro CRISPR. ( A ) The specific products of the in vitro Otc CRISPR digestion of both YAC ADK.A6 DNA and mouse genomic DNA are resolved by PFGE and detected with Diamond stain (Promega) . Whereas the expected YAC digestion products are clearly detectable, digestion of the more complex mouse genomic DNA does not produce detectable products. Untreated and Cas9-only digestions of YAC and mouse genomic DNA serve as negative controls. ( B ) A Southern blot of the PFG shown in (A) is hybridized with a DIG-labeled Otc probe. The intact YAC chromosome (∼520 kb) and the expected 263 kb in vitro Otc CRISPR digestion product from both YAC and mouse genomic DNA are visible upon chemiluminescent detection of the probe and exposure of X-ray film. ( C ) A faint but, detectable ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is resolved by PFGE along with the multi-megabase H. wingei (Hw) chromosomes ladder (Bio-Rad) labeled on the left. ( D ) The Southern blot of the PFG shown in ( C ) is hybridized with a DIG-labeled Srsx probe. The estimated ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is visible upon chemiluminescent detection of the probe and exposure of X-ray film.

    Journal: Nucleic Acids Research

    Article Title: CRISPR-mediated isolation of specific megabase segments of genomic DNA

    doi: 10.1093/nar/gkx749

    Figure Lengend Snippet: Large segments of the mouse X chromosome are specifically targeted with in vitro CRISPR. ( A ) The specific products of the in vitro Otc CRISPR digestion of both YAC ADK.A6 DNA and mouse genomic DNA are resolved by PFGE and detected with Diamond stain (Promega) . Whereas the expected YAC digestion products are clearly detectable, digestion of the more complex mouse genomic DNA does not produce detectable products. Untreated and Cas9-only digestions of YAC and mouse genomic DNA serve as negative controls. ( B ) A Southern blot of the PFG shown in (A) is hybridized with a DIG-labeled Otc probe. The intact YAC chromosome (∼520 kb) and the expected 263 kb in vitro Otc CRISPR digestion product from both YAC and mouse genomic DNA are visible upon chemiluminescent detection of the probe and exposure of X-ray film. ( C ) A faint but, detectable ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is resolved by PFGE along with the multi-megabase H. wingei (Hw) chromosomes ladder (Bio-Rad) labeled on the left. ( D ) The Southern blot of the PFG shown in ( C ) is hybridized with a DIG-labeled Srsx probe. The estimated ∼2.3 Mb in vitro Srsx CRISPR digestion product from mouse genomic DNA is visible upon chemiluminescent detection of the probe and exposure of X-ray film.

    Article Snippet: Size ladders of yeast chromosomes or the Yeast Chromosome PFG Markers (Bio-Rad) are loaded on all megabase resolution gels.

    Techniques: In Vitro, CRISPR, Staining, Southern Blot, Labeling